Novel, high incidence exercise-induced muscle bleeding model in hemophilia B mice: rationale, development and prophylactic intervention

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Novel, high incidence exercise-induced muscle bleeding model in hemophilia B mice : rationale, development and prophylactic intervention. / Tranholm, M.; Kristensen, Annemarie Thuri; Broberg, M. L.; Groth, Mette Perch.

In: Journal of Thrombosis and Haemostasis, Vol. 13, No. 1, 2015, p. 82-91.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tranholm, M, Kristensen, AT, Broberg, ML & Groth, MP 2015, 'Novel, high incidence exercise-induced muscle bleeding model in hemophilia B mice: rationale, development and prophylactic intervention', Journal of Thrombosis and Haemostasis, vol. 13, no. 1, pp. 82-91. https://doi.org/10.1111/jth.12775

APA

Tranholm, M., Kristensen, A. T., Broberg, M. L., & Groth, M. P. (2015). Novel, high incidence exercise-induced muscle bleeding model in hemophilia B mice: rationale, development and prophylactic intervention. Journal of Thrombosis and Haemostasis, 13(1), 82-91. https://doi.org/10.1111/jth.12775

Vancouver

Tranholm M, Kristensen AT, Broberg ML, Groth MP. Novel, high incidence exercise-induced muscle bleeding model in hemophilia B mice: rationale, development and prophylactic intervention. Journal of Thrombosis and Haemostasis. 2015;13(1):82-91. https://doi.org/10.1111/jth.12775

Author

Tranholm, M. ; Kristensen, Annemarie Thuri ; Broberg, M. L. ; Groth, Mette Perch. / Novel, high incidence exercise-induced muscle bleeding model in hemophilia B mice : rationale, development and prophylactic intervention. In: Journal of Thrombosis and Haemostasis. 2015 ; Vol. 13, No. 1. pp. 82-91.

Bibtex

@article{6ecfcf4a47aa4aa8abdcfb38e7d53a4d,
title = "Novel, high incidence exercise-induced muscle bleeding model in hemophilia B mice: rationale, development and prophylactic intervention",
abstract = "INTRODUCTION: Muscle hematomas are the second most common complication of hemophilia and insufficient treatment may result in serious and even life-threatening complications. Hemophilic dogs and rats do experience spontaneous muscle bleeding, but currently, no experimental animal model is available specifically investigating spontaneous muscle bleeds in a hemophilic setting.AIM: The objective of this study was to develop a model of spontaneous muscle bleeds in hemophilia B mice. We hypothesized that treadmill exercise would induce muscle bleeds in hemophilia B mice but not in normal non-hemophilic mice and that treatment with recombinant factor IX (rFIX) before treadmill exercise could prevent the occurrence of pathology.METHODS: A total of 203 mice (123 F9-KO and 80 C57BL/6NTac) were included in three separate studies: (i) the model implementation study investigating the bleeding pattern in hemophilia B mice after treadmill exercise; (ii) a study evaluating the pharmacokinetics of recombinant FIX (rFIX) in hemophilia B mice and based on these data; (iii) the treatment study, which tested therapeutic intervention with rFIX. At termination of the treadmill studies the presence of bleeds was evaluated.RESULTS: Treadmill exercise resulted in a high incidence of muscle bleeds in F9-KO mice but not in C57BL/6NTac mice. Treating hemophilia B mice with rFIX before treadmill exercise prevented muscle bleeds.CONCLUSION: A novel model of muscle bleeds in hemophilia B mice, responsive to rFIX, has been developed.",
author = "M. Tranholm and Kristensen, {Annemarie Thuri} and Broberg, {M. L.} and Groth, {Mette Perch}",
note = "{\textcopyright} The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals, Inc. on behalf of International Society on Thrombosis and Haemostasis.",
year = "2015",
doi = "10.1111/jth.12775",
language = "English",
volume = "13",
pages = "82--91",
journal = "Journal of Thrombosis and Haemostasis",
issn = "1538-7933",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Novel, high incidence exercise-induced muscle bleeding model in hemophilia B mice

T2 - rationale, development and prophylactic intervention

AU - Tranholm, M.

AU - Kristensen, Annemarie Thuri

AU - Broberg, M. L.

AU - Groth, Mette Perch

N1 - © The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals, Inc. on behalf of International Society on Thrombosis and Haemostasis.

PY - 2015

Y1 - 2015

N2 - INTRODUCTION: Muscle hematomas are the second most common complication of hemophilia and insufficient treatment may result in serious and even life-threatening complications. Hemophilic dogs and rats do experience spontaneous muscle bleeding, but currently, no experimental animal model is available specifically investigating spontaneous muscle bleeds in a hemophilic setting.AIM: The objective of this study was to develop a model of spontaneous muscle bleeds in hemophilia B mice. We hypothesized that treadmill exercise would induce muscle bleeds in hemophilia B mice but not in normal non-hemophilic mice and that treatment with recombinant factor IX (rFIX) before treadmill exercise could prevent the occurrence of pathology.METHODS: A total of 203 mice (123 F9-KO and 80 C57BL/6NTac) were included in three separate studies: (i) the model implementation study investigating the bleeding pattern in hemophilia B mice after treadmill exercise; (ii) a study evaluating the pharmacokinetics of recombinant FIX (rFIX) in hemophilia B mice and based on these data; (iii) the treatment study, which tested therapeutic intervention with rFIX. At termination of the treadmill studies the presence of bleeds was evaluated.RESULTS: Treadmill exercise resulted in a high incidence of muscle bleeds in F9-KO mice but not in C57BL/6NTac mice. Treating hemophilia B mice with rFIX before treadmill exercise prevented muscle bleeds.CONCLUSION: A novel model of muscle bleeds in hemophilia B mice, responsive to rFIX, has been developed.

AB - INTRODUCTION: Muscle hematomas are the second most common complication of hemophilia and insufficient treatment may result in serious and even life-threatening complications. Hemophilic dogs and rats do experience spontaneous muscle bleeding, but currently, no experimental animal model is available specifically investigating spontaneous muscle bleeds in a hemophilic setting.AIM: The objective of this study was to develop a model of spontaneous muscle bleeds in hemophilia B mice. We hypothesized that treadmill exercise would induce muscle bleeds in hemophilia B mice but not in normal non-hemophilic mice and that treatment with recombinant factor IX (rFIX) before treadmill exercise could prevent the occurrence of pathology.METHODS: A total of 203 mice (123 F9-KO and 80 C57BL/6NTac) were included in three separate studies: (i) the model implementation study investigating the bleeding pattern in hemophilia B mice after treadmill exercise; (ii) a study evaluating the pharmacokinetics of recombinant FIX (rFIX) in hemophilia B mice and based on these data; (iii) the treatment study, which tested therapeutic intervention with rFIX. At termination of the treadmill studies the presence of bleeds was evaluated.RESULTS: Treadmill exercise resulted in a high incidence of muscle bleeds in F9-KO mice but not in C57BL/6NTac mice. Treating hemophilia B mice with rFIX before treadmill exercise prevented muscle bleeds.CONCLUSION: A novel model of muscle bleeds in hemophilia B mice, responsive to rFIX, has been developed.

U2 - 10.1111/jth.12775

DO - 10.1111/jth.12775

M3 - Journal article

C2 - 25370152

VL - 13

SP - 82

EP - 91

JO - Journal of Thrombosis and Haemostasis

JF - Journal of Thrombosis and Haemostasis

SN - 1538-7933

IS - 1

ER -

ID: 131363246