Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats
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Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats. / Sørensen, H B; Kristensen, A T; Ravn, H B; Fuglsang, J; Hjortdal, V E.
In: Microsurgery, Vol. 19, No. 8, 1999, p. 369-73.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats
AU - Sørensen, H B
AU - Kristensen, A T
AU - Ravn, H B
AU - Fuglsang, J
AU - Hjortdal, V E
N1 - Copyright 1999 Wiley-Liss, Inc.
PY - 1999
Y1 - 1999
N2 - Thrombosis is still a significant problem in microvascular surgery. The aim of this study was to evaluate the antithrombotic effect of topically applied active site-inhibited recombinant human factor VIIa (FFR-rFVIIa) in a rat model with microvascular thrombosis. Forty-five male rats were allocated to one of three groups: local treatment with vehicle only, local treatment with 0.035 mg of FFR-rFVIIa, or local treatment with 0.35 mg of FFR-rFVIIa. An arteriotomy was made in the right femoral artery. Ten minutes following topical application, a thrombogenic anastomosis was performed. Using a transilluminator, thrombus formation and anastomotic bleeding episodes were observed and registered for 40 min. Local application of FFR-rFVIIa resulted in a 85-90% reduction of thrombus formation in both treated groups compared to the control group, but the reduction was only statistically significant in the group treated with 0.035 mg of FFR-rFVIIa. An increased occurrence and duration of anastomotic bleeding episodes were observed in both FFR-rFVIIa-treated groups.
AB - Thrombosis is still a significant problem in microvascular surgery. The aim of this study was to evaluate the antithrombotic effect of topically applied active site-inhibited recombinant human factor VIIa (FFR-rFVIIa) in a rat model with microvascular thrombosis. Forty-five male rats were allocated to one of three groups: local treatment with vehicle only, local treatment with 0.035 mg of FFR-rFVIIa, or local treatment with 0.35 mg of FFR-rFVIIa. An arteriotomy was made in the right femoral artery. Ten minutes following topical application, a thrombogenic anastomosis was performed. Using a transilluminator, thrombus formation and anastomotic bleeding episodes were observed and registered for 40 min. Local application of FFR-rFVIIa resulted in a 85-90% reduction of thrombus formation in both treated groups compared to the control group, but the reduction was only statistically significant in the group treated with 0.035 mg of FFR-rFVIIa. An increased occurrence and duration of anastomotic bleeding episodes were observed in both FFR-rFVIIa-treated groups.
KW - Administration, Topical
KW - Animals
KW - Arteriovenous Anastomosis
KW - Factor VII/administration & dosage
KW - Factor VIIa
KW - Fibrinolytic Agents/administration & dosage
KW - Humans
KW - Male
KW - Microsurgery
KW - Postoperative Hemorrhage/etiology
KW - Rats
KW - Rats, Wistar
KW - Recombinant Proteins/administration & dosage
KW - Thrombosis/prevention & control
U2 - 10.1002/(sici)1098-2752(1999)19:8<369::aid-micr4>3.0.co;2-b
DO - 10.1002/(sici)1098-2752(1999)19:8<369::aid-micr4>3.0.co;2-b
M3 - Journal article
C2 - 10594911
VL - 19
SP - 369
EP - 373
JO - International Journal of Microsurgery
JF - International Journal of Microsurgery
SN - 0738-1085
IS - 8
ER -
ID: 243520873