CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH INCREASED PLATELET LEUKOCYTE AGGREGATION MEASURED BY FLOW CYTOMETRY

Research output: Contribution to journal › Conference abstract in journal › Research › peer-review

Standard

CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH INCREASED PLATELET LEUKOCYTE AGGREGATION MEASURED BY FLOW CYTOMETRY. / Tarnow, Inge; Andreasen, Susanne SH; Olsen, Lisbeth Høier; Moesgaard, Sophia Gry; Rasmussen, Caroline Elisabeth; Kristensen, Annemarie Thuri; Falk, Bo Torkel.

In: Journal of Veterinary Internal Medicine, Vol. 24, No. 3, 06.2010, p. 673.

Research output: Contribution to journal › Conference abstract in journal › Research › peer-review

Harvard

Tarnow, I, Andreasen, SSH, Olsen, LH, Moesgaard, SG, Rasmussen, CE, Kristensen, AT & Falk, BT 2010, 'CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH INCREASED PLATELET LEUKOCYTE AGGREGATION MEASURED BY FLOW CYTOMETRY', Journal of Veterinary Internal Medicine, vol. 24, no. 3, pp. 673.

APA

Tarnow, I., Andreasen, S. SH., Olsen, L. H., Moesgaard, S. G., Rasmussen, C. E., Kristensen, A. T., & Falk, B. T. (2010). CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH INCREASED PLATELET LEUKOCYTE AGGREGATION MEASURED BY FLOW CYTOMETRY. Journal of Veterinary Internal Medicine, 24(3), 673.

Vancouver

Tarnow I, Andreasen SSH, Olsen LH, Moesgaard SG, Rasmussen CE, Kristensen AT et al. CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH INCREASED PLATELET LEUKOCYTE AGGREGATION MEASURED BY FLOW CYTOMETRY. Journal of Veterinary Internal Medicine. 2010 Jun;24(3):673.

Author

Tarnow, Inge ; Andreasen, Susanne SH ; Olsen, Lisbeth Høier ; Moesgaard, Sophia Gry ; Rasmussen, Caroline Elisabeth ; Kristensen, Annemarie Thuri ; Falk, Bo Torkel. / CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH INCREASED PLATELET LEUKOCYTE AGGREGATION MEASURED BY FLOW CYTOMETRY. In: Journal of Veterinary Internal Medicine. 2010 ; Vol. 24, No. 3. pp. 673.

Bibtex

@article{9c2f6e4c86b646969eb8859c54d7b365,
title = "CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH INCREASED PLATELET LEUKOCYTE AGGREGATION MEASURED BY FLOW CYTOMETRY",
abstract = "CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH ENHANCED PLATELET-LEUKOCYTE AGGREGATES - A MARKER FOR PLATELET ACTIVATION. I Tarnow1, LH Olsen2, SHS Andreasen2, SG Moesgaard2, CE Rasmussen2, AT Kristensen1, T Falk2. 1Departments of Small Animal Clinical Sciences and 2Animal and Veterinary Basic Sciences, Faculty of Life Science, University of Copenhagen, Denmark. Chronic congestive heart failure (CHF) in humans is associated with abnormal hemostasis, and changes in hemostatic biomarkers carry a poor prognosis. CHF in dogs has been associated with plasma markers of hypercoagulability, however, platelet activation markers have not been investigated in dogs with clinical signs of heart disease. We hypothesized that platelet surface activation markers are higher in dogs with CHF compared to age-matched controls without clinical signs of heart failure. Dogs with compensated congestive heart failure (n 5 21; age 9.9 _ 2.8 years) caused by acquired heart disease and age-matched control dogs (n 5 19; age 9.5 _ 2.0 years) without signs of heart failure were studied in parallel on same days. Platelet surface P-selectin (CD62P binding) expression, activation of platelet surface GPIIb-IIIa (CAP1 binding),monocyte-platelet (MPAs) and neutrophil-platelet aggregates (NPAs) defined as leukocytes positive for CD61 were measured by whole blood flow cytometry asmarkers of platelet activation. For every dog platelet activation was assessed in unstimulated samples, and in response to exogenously added adenosine diphosphate (ADP), thrombin and a synthetic thromboxane A2 analogue 1 epinephrine. Complete blood count and biochemical screening were performed in all dogs. Dogs with CHF had higher MPAs (p 5 0.014) and NPAs ( p 5 0.021) compared to controls (2-way analysis of variance for repeated measurements). ",
author = "Inge Tarnow and Andreasen, {Susanne SH} and Olsen, {Lisbeth H{\o}ier} and Moesgaard, {Sophia Gry} and Rasmussen, {Caroline Elisabeth} and Kristensen, {Annemarie Thuri} and Falk, {Bo Torkel}",
year = "2010",
month = jun,
language = "English",
volume = "24",
pages = "673",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - ABST

T1 - CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH INCREASED PLATELET LEUKOCYTE AGGREGATION MEASURED BY FLOW CYTOMETRY

AU - Tarnow, Inge

AU - Andreasen, Susanne SH

AU - Olsen, Lisbeth Høier

AU - Moesgaard, Sophia Gry

AU - Rasmussen, Caroline Elisabeth

AU - Kristensen, Annemarie Thuri

AU - Falk, Bo Torkel

PY - 2010/6

Y1 - 2010/6

N2 - CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH ENHANCED PLATELET-LEUKOCYTE AGGREGATES - A MARKER FOR PLATELET ACTIVATION. I Tarnow1, LH Olsen2, SHS Andreasen2, SG Moesgaard2, CE Rasmussen2, AT Kristensen1, T Falk2. 1Departments of Small Animal Clinical Sciences and 2Animal and Veterinary Basic Sciences, Faculty of Life Science, University of Copenhagen, Denmark. Chronic congestive heart failure (CHF) in humans is associated with abnormal hemostasis, and changes in hemostatic biomarkers carry a poor prognosis. CHF in dogs has been associated with plasma markers of hypercoagulability, however, platelet activation markers have not been investigated in dogs with clinical signs of heart disease. We hypothesized that platelet surface activation markers are higher in dogs with CHF compared to age-matched controls without clinical signs of heart failure. Dogs with compensated congestive heart failure (n 5 21; age 9.9 _ 2.8 years) caused by acquired heart disease and age-matched control dogs (n 5 19; age 9.5 _ 2.0 years) without signs of heart failure were studied in parallel on same days. Platelet surface P-selectin (CD62P binding) expression, activation of platelet surface GPIIb-IIIa (CAP1 binding),monocyte-platelet (MPAs) and neutrophil-platelet aggregates (NPAs) defined as leukocytes positive for CD61 were measured by whole blood flow cytometry asmarkers of platelet activation. For every dog platelet activation was assessed in unstimulated samples, and in response to exogenously added adenosine diphosphate (ADP), thrombin and a synthetic thromboxane A2 analogue 1 epinephrine. Complete blood count and biochemical screening were performed in all dogs. Dogs with CHF had higher MPAs (p 5 0.014) and NPAs ( p 5 0.021) compared to controls (2-way analysis of variance for repeated measurements).

AB - CONGESTIVE HEART FAILURE IN DOGS IS ASSOCIATED WITH ENHANCED PLATELET-LEUKOCYTE AGGREGATES - A MARKER FOR PLATELET ACTIVATION. I Tarnow1, LH Olsen2, SHS Andreasen2, SG Moesgaard2, CE Rasmussen2, AT Kristensen1, T Falk2. 1Departments of Small Animal Clinical Sciences and 2Animal and Veterinary Basic Sciences, Faculty of Life Science, University of Copenhagen, Denmark. Chronic congestive heart failure (CHF) in humans is associated with abnormal hemostasis, and changes in hemostatic biomarkers carry a poor prognosis. CHF in dogs has been associated with plasma markers of hypercoagulability, however, platelet activation markers have not been investigated in dogs with clinical signs of heart disease. We hypothesized that platelet surface activation markers are higher in dogs with CHF compared to age-matched controls without clinical signs of heart failure. Dogs with compensated congestive heart failure (n 5 21; age 9.9 _ 2.8 years) caused by acquired heart disease and age-matched control dogs (n 5 19; age 9.5 _ 2.0 years) without signs of heart failure were studied in parallel on same days. Platelet surface P-selectin (CD62P binding) expression, activation of platelet surface GPIIb-IIIa (CAP1 binding),monocyte-platelet (MPAs) and neutrophil-platelet aggregates (NPAs) defined as leukocytes positive for CD61 were measured by whole blood flow cytometry asmarkers of platelet activation. For every dog platelet activation was assessed in unstimulated samples, and in response to exogenously added adenosine diphosphate (ADP), thrombin and a synthetic thromboxane A2 analogue 1 epinephrine. Complete blood count and biochemical screening were performed in all dogs. Dogs with CHF had higher MPAs (p 5 0.014) and NPAs ( p 5 0.021) compared to controls (2-way analysis of variance for repeated measurements).

M3 - Conference abstract in journal

VL - 24

SP - 673

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

IS - 3

ER -

ID: 32648014